Affiliation:
1. Laboratorio de Biología del Desarrollo - Instituto Tecnológico de Chascomús. INTECH (CONICET-UNSAM), Argentina
2. Institut national de la recherche scientifique (INRS) - Centre Eau Terre Environnement, Quebec, Canada
3. Laboratorio de Ictiofisiología y Acuicultura - INTECH (CONICET-UNSAM), Argentina
Abstract
Exposure to environmental stressors, like high temperature (HT), during early development of fish induces sex reversal of genotypic females. Nevertheless, the involvement of the brain in this process is not well clarified. In the present work, we investigated the mRNA levels of corticotropin-releasing hormone b (crhb) and its receptors (crhr1 and crhr2), and found out that they were up-regulated at HT during the critical period of gonadal sex determination in medaka. In order to clarify their roles in sex reversal, biallelic mutants for crhr1 and crhr2 were produced by CRISPR/Cas9 technology. Remarkably, biallelic mutant of both loci (crhr1 and crhr2) did not undergo female-to-male sex reversal upon HT exposition. Inhibition of this process in double crhrs mutants could be successfully rescued through the administration of the downstream effector of the hypothalamic-pituitary interrenal axis, the cortisol. Taken together, these results revealed for the first time the participation of the CNS acting as a transducer of masculinization induced by thermal stress.
Funder
Agencia Nacional de Promoci?n Cient?fica y Tecnol?gica
Consejo Nacional de Investigaciones Cient?ficas y T?cnicas
Natural Sciences and Engineering Research Council of Canada
Canada Research Chairs
Publisher
The Company of Biologists
Subject
Developmental Biology,Molecular Biology
Cited by
40 articles.
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