Author:
Stroud Matthew J,Nazgiewicz Alicja,McKenzie Edward A,Wang Yisu,Kammerer Richard A,Ballestrem Christoph
Abstract
Crosstalk between the microtubule (MT) and actin cytoskeletons is fundamental to many cellular processes including cell polarisation and cell motility. Previous work has shown that members of the growth arrest specific 2 (GAS2) family mediate the crosstalk between filamentous actin (F-actin) and MTs, but the molecular basis remains unclear. By using fluorescence microscopy, we demonstrate that three members of this family, GAS2-like 1 (G2L1), GAS2-like 2 (G2L2), and GAS2-like 3 (G2L3) are differentially involved in mediating the crosstalk between F-actin and MTs. While all localise to actin and MTs, only the exogenous expression of G2L1 and G2L2 influenced MT stability, dynamics, and guidance along actin stress fibres. Biochemical analysis and live-cell imaging reveal that their functions are largely due to the association of these proteins with MT plus-end binding (EB) proteins that bind to SxIP/SxLP motifs located at G2L C-termini. Our findings lead to a model in which EB proteins play a key role in mediating actin-MT crosstalk.
Publisher
The Company of Biologists
Cited by
51 articles.
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