Approbation of the Technology for Constructing Means of Express Indication of New Especially Dangerous

Author:

Petrov A. A.1,Kazantsev A. V.1,Panferov K. A.1,Chislov A. A.1,Kovalchuk E. A.1,Kutaev D. A.1,Borisevich S. V.1ORCID

Affiliation:

1. Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defense

Abstract

Catastrophic pandemic of the particularly dangerous coronavirus SARS-CoV-2 in 2020–2022 and the unexpected  spread of the monkeypox pathogen from Africa in 2022, demonstrate the need for an adequate response to biological  threats that have exotic infections as their source, overcome the interspecies barrier between animals and humans  and have high rates of virulence and contagiousness in relation to the latter. The purpose of the article is to create  a technology for constructing means for the express indication of new especially dangerous and exotic infections,  which makes it possible to quickly develop a gene diagnostic tool, evaluate its characteristics and launch large-scale  production. Materials and methods. The authors used technologies for constructing means for express indication  of new especially dangerous and exotic infections based on real-time polymerase chain reaction (RT-PCR-RT-Flu/ Coronavirus) methods, suitable for multiplex identification of coronavirus RNA. The discussion of the results. The  developed technology for constructing means for express indication of new especially dangerous and exotic infections  was successfully tested at the laboratory base of the FSBЕ «48 Central Research Institute» of the Ministry of Defense  of the Russian Federation using the example of designing a «Set of reagents for detecting the RNA of coronaviruses  SARS-CoV, MERS-CoV, SARS-CoV-2 and virus influenza A by real-time polymerase chain reaction (RT-PCR-RT-Flu/Coronavirus)», suitable for multiplex identification of coronavirus RNA. Conclusion. As a result of the research  carried out to evaluate the equipment available at the laboratory base of the FSBЕ «48 Central Research Institute» of  the Ministry of Defense of the Russian Federation, the adaptation and implementation of key production processes,  the development and production of express-indication reagents, as well as testing the technology for constructing  express-indication means for new especially dangerous and exotic infections, using the example of designing a set of  RT-PCR-RV-Flu/Coronavirus reagents, a gene diagnostic platform was created for the development of reagents for the  express indication of new especially dangerous and exotic infections.

Publisher

27 Science Center

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