Character of β-lymphocytes differentiation in women with hypertensive disorders during pregnancy

Author:

Panova I. A.1ORCID,Kudryashova A. V.1ORCID,Panashchatenko A. S.1ORCID,Rokotyanskaya E. A.1ORCID,Malyshkina A. I.1ORCID,Parejshvili V. V.1ORCID,Harlamova N. V.1ORCID

Affiliation:

1. «Ivanovo Scientific Research Institute of Maternity and Childhood by V.N. Gorodkov» of the Russian Ministry of health

Abstract

The aim of the work was to identify the features of B-lymphocyte differentiation in women with hypertensive disorders of various origins, to establish their relationship with indicators of the elastic properties of the vascular wall, and to develop additional diagnostic criteria for the severity of preeclampsia. We examined 193 women at 24-40 weeks of gestation. Of these, 39 women with chronic arterial hypertension, 35 women with preeclampsia that developed against the background of chronic arterial hypertension, 55 with preeclampsia. The control group consisted of 64 women without hypertensive disorders. To assess the elastic properties of the vascular wall, a sphygmographic attachment of the “Poly-Spectrum-8” hardware-software complex (“Neurosoft” Ivanovo) was used. The relative content of B-lymphocytes (CD19 + and CD20 +), regulatory B-cells (CD20 + IL-10 +), switched (CD19 + CD27 + IgD-) and non-switched (CD19 + CD27 + IgD +) memory cells, plasma cells (CD19 + CD20-CD38 +) in the general population of B-lymphocytes was assessed by flow cytometry on a FACSCantoII flow cytometer using the FACS Diva program. Statistical analysis was carried out using the programs “Statistica for Windows 6.0”, “Microsoft Excel 2010” and “MedCals”. All subjects with hypertensive disorders showed an increase in the stiffness of the arteries of the muscular and elastic types, the most pronounced in the groups of patients with PE, the maximum in women with CAH and associated PE. An increased level of B1-lymphocytes in the peripheral blood is also noted in all hypertensive disorders. There were revealed positive correlations of high strength between: the level of CD20 + cells and the velocity of pulse wave propagation through the arteries of the muscular type (PWVm) in all groups with hypertensive disorders; the content of B1 cells and PWVM in moderate preeclampsia; level emory B-cells and PWV in elastic-type arteries in women with CAH and associated PE. The ROC analysis of the relative content of B1 cells in the general population of B lymphocytes and the content of IL-10-producing cells in the population of CD20 + lymphocytes (Breg) in women with moderate and severe PE revealed criteria for the differential diagnosis of preeclampsia of varying severity. The presence of hypertensive disorders of various origins is accompanied by a decrease in the elasticity of the arterial vascular wall, which is most pronounced in patients with CAH and associated PE. These changes are largely correlated with the level of B cells. As additional criteria for determining the severity of PE, the relative content of B1 cells and IL-10-producing cells in the population of Breg CD20 + lymphocytes can be used.

Publisher

EKOlab

Subject

Biochemistry (medical),Medical Laboratory Technology,General Medicine

Reference33 articles.

1. Ajlamazyan E.K., Repina M.A. Comments on the clinical protocol «Hypertension during pregnancy, preeclampsia, eclampsia». Zhurnal akusherstva i zhenskikh bolezney. 2012; 5: 3–9. (in Russian)

2. Baturina I.L., Abramovskih O.S., Loginova Yu.V., Nikushkina K.V., Zotova M.A. et al. Analysis of T cyt-lymphocyte differentiation in recurrent miscarriage. Meditsinskaya immunologiya. Spetsial’nyj vypusk.2017;19:177. (in Russian)

3. Verlohren S., Muller D.N., Luft F.C., Dechend R. Immunology in hypertension, preeclampsia, and target-organ damage. Hypertension. 2009; 54(3):439-43.

4. Panova I. A., Malyshkina A.I., Kudrjashova A. V., Hlipunova D. A., Rokotjanskaja E. A., Sytova L. A. Synthesis of matrix metalloproteinases and their inhibitors by peripheral phagocytes in pregnant women with hypertensive disorders. Zhurnal akusherstva i zhenskikh bolezney. 2015; 3 :26-32. (in Russian)

5. Granger Joey P. Inflammatory cytokines, vascular function, and hypertension. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2004; 286 (6): 989 – 90.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3