Abstract
Quality control (QC) analysis is an integral part of nucleic acid and CRISPR applications. Utilizing QC checkpoints at critical junctures in the CRISPR process eliminates unsuitable samples, saves time and money, and maximizes resources. This in turn increases confidence in results and builds consistency in the workflow. Advanced Analytical Technologies, Inc. provides reliable and consistent quality analysis with parallel capillary electrophoresis (CE) instruments. CE instruments provide extreme sensitivity and resolution that make them ideal for QC in CRISPR experiments, from detection of minimal degradation in sgRNA to detection of dsDNA starting product when generating ssDNA end-product for HDR, to determining the length of polyadenylated Cas9 mRNA.
Subject
General Biochemistry, Genetics and Molecular Biology,Biotechnology