UV-SPECTROPHOTOMETRIC DETERMINATION OF PARTITION COEFFICIENT (LOG P) OF OLEANOLIC ACID AND ITS COMPARISON WITH SOFTWARE PREDICTED VALUES

Abstract

Oleanolic acid is reported to have various pharmacological activities of therapeutic interest. In order to use it in modern therapy, it is necessary to understand about its drug designing parameters. The current study deals with the determination of partition coefficient (log p) of oleanolic acid by UV-spectrophotometer and its comparison with the values predicted by the online software. For the determination of partition coefficient of oleanolic acid, the shake-flask method was used in which the drug is allowed to partition between n-octanol and distilled water. Further, the amount of drug solubilized in the n-octanol was determined using a UV-spectrophotometer. The partition coefficient of oleanolic acid obtained by said method was 5.32, while, the online software predicted its log p values ranging from 3.94 -7.49. The log p value of oleanolic acid obtained by experiment and predicted by the online software concluded that the said molecule is lipophilic in nature.