Affiliation:
1. Department of Clinical Immunology, Medical University of Warsaw, Warsaw, Poland
2. Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland
3. Bacteriophage Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland
Abstract
Introduction: Bacteriophages are an abundant component of the mucosal microbiota in humans and some
animal species. Intestinal epithelial cells (IECs) are the key element responsible for the induction
and regulation of immune responses in the gut mucosa. The objective of this study was
to evaluate the effects of T4 and A5/80 bacteriophages on the expression of immunologically
important genes in Caco-2, a model cell line for IECs.
Materials & Method: Bacteriophages were added to cultures of differentiated Caco-2 cells for 12 hours, while
control cultures were treated with phosphate-buffered saline (PBS). Expression of genes in
Caco-2 cells was determined using custom-made RT2 Profiler PCR Arrays, which allow for
the evaluation of gene expression with the sensitivity and specificity of real-time PCR. We
evaluated the expression of 21 genes which are important for the immune functions of IECs,
including IL1B, IL6, IL7, IL10, IL15, IL18, IL25, IL33, TGFB1, TNF, CXCL8, CCL2, TSLP, FCER2, PIGR, DEFB4A,
CAMP, REG3G, TNFSF13, TNFSF13B, and MUC2.
Results: Both examined phages significantly influenced the expression of a number of genes compared
with control cultures. In particular, T4 significantly increased the expression of the CCL2 and
DEFB4A genes, while A5/80 induced the expression of the PIGR gene.
Discussion: Together with the findings from previous studies, our results suggest that by modulating the
expression of some genes, bacteriophages may affect immune responses in the gut mucosa.
Subject
Infectious Diseases,Microbiology (medical)
Cited by
5 articles.
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