Affiliation:
1. Slovak University of Agriculture in Nitra AgroBioTech Research Centre
2. Slovak University of Agriculture in Nitra Faculty of Agrobiology and Food Resources Institute of Plant and Environmental Sciences
Abstract
Several types of allergies are currently known and are characterized by an exaggerated response of the immune system to substances from various sources called allergens. One of them is a food allergy, which is becoming more common in the population. For this reason, it is necessary to describe the issue from several aspects including genomic variability of plant allergens. The objective of this study was to analyse intraspecific variability of Bet v 1 of 10 different varieties of apple species (Malus domestica Borkh.). BBAP technique for genomic determination of the presence of Bet v 1 homologs at the DNA level was performed. Degenerate primers that anneal a variable and conserved part of PR-10 protein homologues genes were used in the analyse. Amplicons were generated and formed relatively monomorphic profiles, indicating the stability of the given isoforms of Bet v 1 within the selected apple varieties. To evaluate the potential allergenicity of selected varieties further studies on another molecular level such as a comparison of gene expression of the PR-10 family members and their protein expression levels are needed.
Publisher
University of Applied Sciences in Tarnow, Poland
Reference27 articles.
1. Phipps J. B., Robertson K. R., Smith P. G., Rohrer J. R.: A checklist of the subfamily Maloideae (Rosaceae); Canadian Journal of Botany. 1990; 68(10):2209-2269. Doi:10.1139/b90-288
2. Janick J., Moore J. N. (Eds.): Fruit breeding, tree and tropical fruits. New York: John Wiley & Sons, 1996. 1-77. ISBN: 978-0-471-31014-3.
3. FAO (Food and Agriculture Organization of the United Nations). 2019; http://www.fao.org
4. Boyer, J., Liu, R. H.: Apple phytochemicals and their health benefits; Nutrition Journal. 2004; 3:5. Doi: 10.1186/1475-2891-3-5
5. Somssich I. E., Schmelzer E., Kawalleck P., Hahlbrock K.: Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsley; Molecular and General Genetics. 1988; 213:93–98. Doi: 10.1007/BF00333403
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献