Study of the correlation between levels of TNF-α and MCP-1 in plasma and tissues of rats infected with pseudomonas aeruginosa

Abstract

Abstract For the importance of the infection with Pseudomonas aeruginosa which result in serious illness and the complications that may exceed more than one organ of the body, this study was carried using laboratory animals, rats as a model for the mammals’ animals to detect the levels of TNF-α and MCP-1 in plasma and tissues and the histopathological effects that can be caused by these bacteria in several organs, lung, liver, spleen, kidney and heart. Methods: In this study, we used 24 rats, 12 untreated (negative) group and 12 treated group that injected intrapertonally with Pseudomonas aeruginosa suspension for two periods (1 and 7 days). At the end of experimental period, animals were sacrificed, blood samples were collected and dissected each of lung, liver, spleen, kidney and heart to determine the level of TNF-α and MCP-1 in the plasma and tissues in addition to investigate the histopathological changes. Results: Rats infection with Pseudomonas aeruginosa caused a significant increase in the levels of TNF-α and MCP-1 in the plasma and studied organs tissues in comparison with negative rats. In addition to investigate the positive correlation between TNF-α and MCP-1 levels in both plasma and tissues. Light microscopic examination appeared the structural changes in all studied organs of animal infected with P. aeruginosa for two periods and this change was more acute in 7 days of treated compared with untreated groups, where have normal structure. In lung, infiltrations of inflammatory cells in addition to necrosis and hemorrhage of respiratory units. Liver sections characterized by irregularity architecture structure with congestion of central vein. Also, we proved abnormal texture of spleen tissue and proliferation in central germination of white pulp. In addition to dilation in parts of urinary duct in kidney, including glomerular collapse, infiltration of lymphocytes. For heart, there is a disorganization of myocardium tissues. While all organs slices of negative groups were normal. Conclusions: We conclude from the results of the current study that the infection with Pseudomonas aeruginosa can elevate inflammation in most organs of the body by induce over expression of TNF-α and MCP-1, which is reflected negatively on the performance of the function and thus the overall health of the body.