Looking at the potential of HSP70 gene sequence in the germplasm of Indonesian green jungle fowl

Abstract

Abstract It has long been known that the concentration of intracellular heat shock proteins will increase following stressful conditions, whether physical stress (heat, pH, osmotic), infection/infestation with parasitic organisms, or stress via neural pathways. The research aimed to characterize the Heat Shock Proteins (HSP) 70 gene in green jungle fowl (GJF) as a basis for germplasm sources in developing chickens resistant to heat stress. The sequence read archive (SRA) of GJF deposited in GenBank originating from Blora (Central Java), Banyuwangi (East Java), and Madura is called SRA data was compiled and aligned to the complete HSP70 gene from broiler chickens. The HSP70 gene segment in GJF is 2320 bp consisting of ‘5UTR (110 bp), CDS (1905 bp/635 aa) and 3’UTR (305 bp). The 5’UTR heterozygous condition was found in GJF Madura and Blora, each at one base position out of 110 bases, while the 3’UTR was found in GJF Madura and Blora also at four base positions out of 305 bases. Two differentiating bases exist between broiler chickens and GJF in the UTR area. Heterozygous conditions in the CDS area have 45 sites (from 1905 bp). The HSP70 sequence found in Indonesian green jungle fowl germplasm, especially in the gene control segments (5’UTR and 3’UTR), revealed promising therapeutic and agronomic potential.