Characteristics of Nano Zn-Fitogenik (NZF) made by green synthesis process using guava leaves (Psidium guajava) for feed additives

Abstract

Abstract The main objective of this study was to characterize (chemical, biological, and physical) of Nano Zn-Fitogenik (NZF) synthesized using guava leaves (P. guajava) extracted using water. Among the stages conducted in the study were extraction of guava leaves, NZF biosynthesis using green synthesis method, and evaluation of NZF characteristics. Parameters detected in the evaluation of the chemical and biological characteristics of the NZF used a completely randomized experimental (CRD) design using 2 main factors (unheated water versus hot water), where each experimental unit was repeated 3 times. The results of this study revealed that chemical characterization study showed that NZF contained 0.15% dry matter (DM) of total phenol, 12.68% DM Zn, and 22.12 mg ml−1 of antioxidant activity (IC50). NZF had the ability as an antibacterial agent against Escherichia coli and Salmonella enteridis. FTIR analysis showed that NZF contained phytogenic compounds sourced from guava leaf extract (Psidium guajava). It can be concluded that NZF is a nanoparticle containing Zn and phytogenic compounds (total phenol) which function as an antioxidant and antibacterial. Therefore, NZF has the potential to be used as a feed additive.