Anticancer potential from ethanol extract of Zanthoxylum acanthopodium DC. seed to against MCF-7 cell line

Abstract

Abstract Andaliman (Zanthoxylum acanthopodium DC.) is a wild plant species from North Sumatera. Studies conducted before show that Z. acantopodium seeds contain high antioxidants. Based on International Agency for Research on Cancer (IARC) data, it is known that in 2012 the highest type of cancer in women in the world is breast cancer (38 per 100 000 women). MCF-7 cell line is one of breast cancer model cell. The purpose of this research is to identify types of secondary metabolites which can be found in Andaliman’s seed extract and to test the ability of these compound in stopping the MCF-7 cell line activity. The method used in this research is an experimental method with different concentration and MTT Assay. Secondary metabolites that has been identified are phenol, saponins, tannins, triterpenes, flavonoids, and alkaloids. The result obtained by using MTT Assay with value IC50 and the absorbance of 550 nm was 221.31 mg L−1 which indicate that ethanol extract of Andaliman’s seed has potential as an anticancer and quite actively affect the inhibition of MCF-7 cell line proliferation. Moreover, the pure extract of Andaliman seed may have a strong effect to inhibit MCF-7 cell line proliferation.