Large-field and fast super-resolution microscopic imaging method based on laser interferometry

Author:

Hao HuORCID,Boxing QianORCID,Huigang Wang,Jia Jia

Abstract

Abstract In recent years, structured illumination microscopy (SIM) has been drawing great attention for both technique development and application. However, conventional SIM, which uses a spatial light modulator (SLM) for fringe projection, often has a limited field of view. To meet the demand for high-throughput microscopic imaging in biomedicine research, a large-field super-resolution (SR) fluorescence microscopic imaging method based on laser interferometry was proposed. The method that combines a two-dimensional (2D) grating for fringe pattern projection and an SLM for selecting fringe orientation can break the limitation of fringe number limited by the digital projection devices. A spatial-domain reconstruction algorithm was developed to improve the computational speed of super-resolution imaging. Finally, an experimental platform for SIM microscopy was established. A large-field view of 1380 μm × 1035 μm under a 20×/NA0.75 objective is experimentally demonstrated, and an enhancement of 1.8-fold resolution is realized. The spatial-domain reconstruction algorithm can significantly improve the computational speed by approximately 10 times faster compared to the traditional frequency-domain algorithm.

Funder

China National Funds for Distinguished Young Scientists

Publisher

IOP Publishing

Reference25 articles.

1. Beitrge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung;Abbe;Arch. Mikr. Anat.,1873

2. Design of super resolution system for single pixel imaging results based on Fourier spectrum acquisition;Hang;J. Electron. Meas. Instrum.,2022

3. Lightweight super-resolution reconstruction method based on hierarchical features fusion and attention mechanism for mine image;Cheng;Chin. J. Sci. Instrum.,2022

4. A long-distance pedestrian small target detection method;Shi;Chin. J. Sci. Instrum.,2022

5. Superresolution by localization of quantum dots using blinking statistics;Lidke;Opt. Express,2005

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3