Affiliation:
1. The Sainsbury Laboratory, University of East Anglia, Norwich NR4 7UH, United Kingdom
2. School of Biosciences, University of Exeter, Exeter EX4 4QD, United Kingdom
Abstract
To cause rice blast disease, the filamentous fungus
Magnaporthe oryzae
secretes a battery of effector proteins into host plant tissue to facilitate infection. Effector-encoding genes are expressed only during plant infection and show very low expression during other developmental stages. How effector gene expression is regulated in such a precise manner during invasive growth by
M. oryzae
is not known. Here, we report a forward-genetic screen to identify regulators of effector gene expression, based on the selection of mutants that show constitutive effector gene expression. Using this simple screen, we identify Rgs1, a regulator of G-protein signaling (RGS) protein that is necessary for appressorium development, as a novel transcriptional regulator of effector gene expression, which acts prior to plant infection. We show that an N-terminal domain of Rgs1, possessing transactivation activity, is required for effector gene regulation and acts in an RGS-independent manner. Rgs1 controls the expression of at least 60 temporally coregulated effector genes, preventing their transcription during the prepenetration stage of development prior to plant infection. A regulator of appressorium morphogenesis is therefore also required for the orchestration of pathogen gene expression required for invasive growth by
M. oryzae
during plant infection.
Funder
EC | ERC | HORIZON EUROPE European Research Council
Gatsby Charitable Foundation
Publisher
Proceedings of the National Academy of Sciences
Cited by
6 articles.
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