Author:
Bewick Adam J.,Ji Lexiang,Niederhuth Chad E.,Willing Eva-Maria,Hofmeister Brigitte T.,Shi Xiuling,Wang Li,Lu Zefu,Rohr Nicholas A.,Hartwig Benjamin,Kiefer Christiane,Deal Roger B.,Schmutz Jeremy,Grimwood Jane,Stroud Hume,Jacobsen Steven E.,Schneeberger Korbinian,Zhang Xiaoyu,Schmitz Robert J.
Abstract
In plants, CG DNA methylation is prevalent in the transcribed regions of many constitutively expressed genes (gene body methylation; gbM), but the origin and function of gbM remain unknown. Here we report the discovery that Eutrema salsugineum has lost gbM from its genome, to our knowledge the first instance for an angiosperm. Of all known DNA methyltransferases, only CHROMOMETHYLASE 3 (CMT3) is missing from E. salsugineum. Identification of an additional angiosperm, Conringia planisiliqua, which independently lost CMT3 and gbM, supports that CMT3 is required for the establishment of gbM. Detailed analyses of gene expression, the histone variant H2A.Z, and various histone modifications in E. salsugineum and in Arabidopsis thaliana epigenetic recombinant inbred lines found no evidence in support of any role for gbM in regulating transcription or affecting the composition and modification of chromatin over evolutionary timescales.
Funder
HHS | NIH | National Institute of General Medical Sciences
Pew Charitable Trusts
National Science Foundation
Damon Runyon Cancer Research Foundation
Howard Hughes Medical Institute
Publisher
Proceedings of the National Academy of Sciences
Cited by
253 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献