Viable offspring derived from single unfertilized mammalian oocytes-Reference-Cited by-全球学者库

Viable offspring derived from single unfertilized mammalian oocytes

Published:2022-03-07 Issue:12 Volume:119 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Wei Yanchang¹²^ORCID,Yang Cai-Rong¹²³,Zhao Zhen-Ao¹²⁴^ORCID

Affiliation:

1. Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200135, China

2. Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200135, China

3. Center for Reproductive Sciences, University of California, San Francisco, CA 94143

4. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Abstract

Significance In mammals, parthenogenesis is limited because of problems arising from genomic imprinting. Here, we report live mammalian offspring derived from single unfertilized eggs. This was achieved by the targeted DNA methylation rewriting of seven imprinting control regions. By designing guide RNAs with protospacer adjacent motif (PAM) sequences matching one allele but not the other, dCas9-Dnmt3a or dCpf1-Tet1 enables targeted DNA methylation editing in an allele-specific manner. The success of parthenogenesis in mammals opens many opportunities in agriculture, research, and medicine.

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Link

https://pnas.org/doi/pdf/10.1073/pnas.2115248119

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