Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.-Reference-Cited by-同舟云学术

Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.

Published:1987-12 Issue:24 Volume:84 Page:8839-8843
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Lue N F¹,Kornberg R D¹

Affiliation:

1. Department of Cell Biology, Stanford University School of Medicine, CA 94305.

Abstract

A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory.

Publisher

Proceedings of the National Academy of Sciences

Link

https://pnas.org/doi/pdf/10.1073/pnas.84.24.8839

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