Transcriptome profiling in swine macrophages infected with African swine fever virus at single-cell resolution-Reference-Cited by-同舟云学术

Transcriptome profiling in swine macrophages infected with African swine fever virus at single-cell resolution

Published:2022-05-04 Issue:19 Volume:119 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Zheng Yuxuan¹,Li Su²,Li Shi-Hua¹,Yu Shaoxiong²^ORCID,Wang Qihui¹³^ORCID,Zhang Kehui²,Qu Liang²,Sun Yuan²,Bi Yuhai¹³,Tang Fuchou⁴⁵⁶,Qiu Hua-Ji²^ORCID,Gao George F.¹³^ORCID

Affiliation:

1. CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Center for Influenza Research and Early-Warning (CASCIRE), Chinese Academy of Sciences (CAS), Beijing 100101, China

2. State Key Laboratory of Veterinary Biotechnology, National High-Containment Laboratory for Animal Diseases Control and Prevention, and National African Swine Fever Para-Reference Laboratory, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China

3. Savaid Medical School, University of the Chinese Academy of Sciences, Beijing 100049, China

4. Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing 100871, China

5. Biomedical Pioneering Innovation Center (BIOPIC), Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Peking University, Beijing 100871, China

6. Peking-Tsinghua Center for Life Sciences (CLS), Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China

Abstract

SignificanceAfrican swine fever virus (ASFV) causes a severe and highly contagious disease in pigs and wild boars, but no commercial vaccines or antivirals are available currently. Understanding the mutual antagonism between virus and host factors during ASFV infection may facilitate the development of new vaccines and antivirals. Our work profiled transcriptomes of swine macrophages infected with ASFV through single-cell RNA-sequencing technology. Identified dynamic transcriptome events of viral genes provide molecular characteristics of ASFV during infection. Moreover, virus–host interactions imply the regulation pathway of viral replication in host cells, which may guide research on antiviral strategies and dissection of ASFV pathogenesis.

Funder

National Key R&D Program of China

National Natural Science Foundation of China

Strategic Priority Research Program of the Chinese Academy of Sciencse

Youth Innovation Promotion Association of the Chinese Academy of Sciences

Intramural Special Grants for African Swine Fever Research from the Chinese Academy of Sciences

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Link

https://pnas.org/doi/pdf/10.1073/pnas.2201288119