Abstract
In insects, the innate immune system is subdivided into cellular and humoral defenses. When parasitoids attack insects, both reactions can be activated and notably, the phenoloxidase (PO) cascade and lytic activity are part of both cellular and humoral defenses. However, to our knowledge, no study has characterized any immune response of the whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) to the attack of Eretmocerus eremicus (Hymenoptera: Aphelinidae). Therefore, the first objective of the present study was to determine whether whitefly nymphs recently parasitized by E. eremicus exhibit any immune response. For this, we estimate the level of prophenoloxidase (proPO), phenoloxidase (PO), and lytic activity by colorimetric assays. A second objective was to assess whether the observed whitefly immune response could be related to a previously reported preference of the predator Geocoris punctipes (Hemiptera: Lygaeidae) for non-parasitized nymphs. We therefore offered non-parasitized and recently parasitized nymphs to the predator. Our results show that parasitism of whitefly nymphs by E. eremicus induced a highly estimated level of proPO and PO, and a lower level of lytic activity. In addition, we found that G. punctipes did not show a preference for non-parasitized over recently parasitized nymphs. The nymphs of T. vaporariorum activated the PO pathway against E. eremicus; however, the increase in proPO and PO levels was traded-off with decreased lytic activity. In addition, the previously reported preference for non-parasitized nymphs was not seen in our experiments, indicating that the induced immune response did not affect predator behavior by G. punctipes.
Funder
CONACyT sabbatical scholarship
PRO-SNI and Fortalecimiento programs
Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica
Publisher
Public Library of Science (PLoS)
Reference83 articles.
1. La evolución de sistemas complejos: El caso del sistema inmune en animales;LF Cadavid;Acta Biol. Colomb,2009
2. Insects hemocytes and their role in immunity;MD Lavine;Insect Biochem. Mol. Biol,2002
3. Exploiting Innate Immunity for Biological Pest Control.;F Liu;Advances in Insect Physiology,2017
4. Peptidoglycan recognition proteins in insect immunity.;Q Wang;Mol. Immunol,2019
5. An overview of insect innate immunity;M Ali Mohammadie Kojour;Entomol. Res,2020
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