Fracture healing on non-union fracture model promoted by non-thermal atmospheric-pressure plasma

Author:

Saito Kosuke,Toyoda HiromitsuORCID,Okada Mitsuhiro,Oh Jun-Seok,Nakazawa Katsumasa,Ban Yoshitaka,Orita Kumi,Shimatani Akiyoshi,Yao Hana,Shirafuji Tatsuru,Nakamura Hiroaki

Abstract

Non-thermal atmospheric-pressure plasma (NTAPP) is attracting widespread interest for use in medical applications. The tissue repair capacity of NTAPP has been reported in various fields; however, little is known about its effect on fracture healing. Non-union or delayed union after a fracture is a clinical challenge. In this study, we aimed to investigate how NTAPP irradiation promotes fracture healing in a non-union fracture model and its underlying mechanism, in vitro and in vivo. For the in vivo study, we created normal and non-union fracture models in LEW/SsNSlc rats to investigate the effects of NTAPP. To create a fracture, a transverse osteotomy was performed in the middle of the femoral shaft. To induce the non-union fracture model, the periosteum surrounding the fracture site was cauterized after a normal fracture model was created. The normal fracture model showed no significant difference in bone healing between the control and NTAPP-treated groups. The non-union fracture model demonstrated that the NTAPP-treated group showed consistent improvement in fracture healing. Histological and biomechanical assessments confirmed the fracture healing. The in vitro study using pre-osteoblastic MC3T3-E1 cells demonstrated that NTAPP irradiation under specific conditions did not reduce cell proliferation but did enhance osteoblastic differentiation. Overall, these results suggest that NTAPP is a novel approach to the treatment of bone fractures.

Funder

JSPS KAKENHI

Glocal hub of wisdom and wellness filled with smiles

Publisher

Public Library of Science (PLoS)

Reference70 articles.

1. Prevalence of long-bone non-unions.;C Tzioupis;Injury.,2007

2. Treatment of long bone non-unions with polytherapy: Indications and clinical results;GM Calori;Injury,2011

3. Quality of life assessment of patients with posttraumatic fracture nonunion, chronic refractory osteomyelitis, and lower-extremity amputation;RK Lerner;Clin Orthop Relat Res,1993

4. Delayed union and nonunions: Epidemiology, clinical issues, and financial aspects.;DJ Hak;Injury,2014

5. Nail Dynamization for Delayed Union and Nonunion in Femur and Tibia Fractures.;J Vaughn;Orthopedics.,2016

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3