Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan

Author:

Litvintseva Anastasia P.ORCID,Bakhiet Sahar,Gade Lalitha,Wagner Darlene D.,Bagal Ujwal R.,Batra Dhwani,Norris Emily,Rishishwar Lavanya,Beer Karlyn D.,Siddig Emmanuel Edwar,Mhmoud Najwa Adam,Chow Nancy A.,Fahal Ahmed

Abstract

Madurella mycetomatis is one of the main causative agents of mycetoma, a debilitating neglected tropical disease. Improved understanding of the genomic diversity of the fungal and bacterial causes of mycetoma is essential to advances in diagnosis and treatment. Here, we describe a high-quality genome assembly of M. mycetomatis and results of the whole genome sequence analysis of 26 isolates from Sudan. We demonstrate evidence of at least seven genetically diverse lineages and extreme clonality among isolates within these lineages. We also performed shotgun metagenomic analysis of DNA extracted from mycetoma grains and showed that M. mycetomatis reads were detected in all sequenced samples with the average of 11,317 reads (s.d. +/- 21,269) per sample. In addition, 10 (12%) of the 81 tested grain samples contained bacterial reads including Streptococcus sp., Staphylococcus sp. and others.

Publisher

Public Library of Science (PLoS)

Subject

Infectious Diseases,Public Health, Environmental and Occupational Health

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