Abstract
PurposesTo evaluate the effects of low frequency whole-body vibration (WBV) on degeneration of articular cartilage and subchondral bone in mice with destabilization of the medial meniscus (DMM)induced osteoarthritis(OA) and mice with normal knee.MethodsTen-week-old C57BL/6J male mice received DMM on right knees, while the left knees performed sham operation. There were six groups: DMM, SHAM DMM, DMM+WBV,SHAM DMM+WBV, DMM+ NON-WBV and SHAM DMM+NON-WBV. After four weeks, the knees were harvested from the DMM and SHAM DMM group. The remaining groups were treated with WBV (10 Hz) or NON-WBV. Four weeks later, the knees were harvested. Genes, containing Aggrecan(Acan) and CollagenⅡ(Col2a1), Matrix Metalloproteinases 3 and 13(MMP3,13), TNFα and IL6, were measured and staining was also performed. OA was graded with OARSI scores, and tibial plateaubone volume to tissue volume ratio(BV/TV), bone surface area to bone volume ratio (BS/BV), trabecular number(Tb.N) and trabecular thickness separation(TS) between groups were analyzed.ResultsIncreased OARSI scores and cartilage degradation were observed after WBV. BV/TV, Tb.N and TS were not significant between the groups. Significant reductions were observed in MMP3, MMP13, Col2a1, Acan, TNFα and IL6 in the DMM+WBV compared to SHAM DMM+WBV group. BV/TV, BS/BV, Tb.N, TS and OARSI scores were not significantly changed in the left knees. IL6 expression in the SHAM DMM+WBV group was significantly increased compared with the SHAM DMM+ NON-WBV group, while Col2a1, Acan and MMP13 expression decreased.ConclusionWBV accelerated cartilage degeneration and caused slight changes in subchondral bone in a DMM-induced OA model. WBV had no morphologic effect on normal joints.
Funder
The National Natural Science Foundation of China
the Project of the Science and Technology Department in Sichuan province
Sichuan Provincial Department of Science and Technology Fund
The Project of the Science and Technology Department in Henan Provincial Department
The Joint project of Henan Provincial Health Commission
The Natural Science Foundation of China-Henan Province
Key Research Projects of Higher Education Institutions in Henan Province
Henan Medical Education Research Project
Publisher
Public Library of Science (PLoS)
Reference70 articles.
1. Osteoarthritis;DJ Hunter;Lancet,2019
2. Epidemiology of knee osteoarthritis in general practice: a registry-based study;D Spitaels;BMJ open,2020
3. Osteoarthritis year in review 2019: biomarkers (biochemical markers).;WE van Spil;Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society.,2020
4. Osteoarthritis year in review 2019: mechanics;MA Hunt;Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society.,2020
5. Subchondral bone remodelling in osteoarthritis;S. Donell;EFORT open reviews,2019