Assessing mycoplasma contamination of cell cultures by qPCR using a set of universal primer pairs targeting a 1.5 kb fragment of 16S rRNA genes

Author:

Jean Audrey,Tardy Florence,Allatif Omran,Grosjean Isabelle,Blanquier Bariza,Gerlier DenisORCID

Funder

Infrastructure BIOBANQUES (INSERM US013) and IBiSA

Publisher

Public Library of Science (PLoS)

Subject

Multidisciplinary

Reference33 articles.

1. Assessing the prevalence of mycoplasma contamination in cell culture via a survey of NCBI's RNA-seq archive;AO Olarerin-George;Nucleic Acids Res,2015

2. Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention;HG Drexler;Cytotechnology,2002

3. Detection of Mycoplasma in cell cultures;L Young;Nat Protoc,2010

4. Multi-primer qPCR assay capable of highly efficient and specific detection of the vast majority of all known Mycoplasma;HK Salling;Biologicals: journal of the International Association of Biological Standardization,2016

5. Characterization of mycoplasmas by PCR and sequence analysis with universal 16S rDNA primers;KE Johansson;Methods in molecular biology,1998

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