Spatiotemporal assessment of pathogenic <i>Leptospira</i> in subtropical coastal watersheds-Reference-Cited by-同舟云学术

Spatiotemporal assessment of pathogenic Leptospira in subtropical coastal watersheds

Published:2024-04-02 Issue:5 Volume:22 Page:923-938
ISSN:1477-8920
Container-title:Journal of Water and Health
language:en
Short-container-title:

Author:

Bridgemohan Ronell¹,Deitch Matthew J.¹,Harmon Emily¹,Whiles Matt R.²,Wilson P. Christopher²,Bean Eban³,Bridgemohan Puran⁴,Bisesi Joseph H.⁵,Nicholas Jodel⁶,Redhead Aaden⁶,Bachoon Dave S.⁶

Affiliation:

1. a Soil, Water, and Ecosystem Sciences Department, University of Florida IFAS West Florida Research and Education Center, Milton, FL, USA

2. b Soil, Water, and Ecosystem Sciences Department 2181 McCarty Hall, University of Florida, P.O. Box 110290, Gainesville, FL 32611, USA

3. c Department of Agricultural and Biological Engineering, University of Florida Main Office, Room 120 Frazier Rogers Hall1741 Museum Road, Building. 474, Gainesville, FL 32611, USA

4. d Tropical Research and Education Center, IFAS Research, University of Florida, Homestead, FL 33031, USA

5. e Department of Environmental and Global Health and Center for Environmental and Human Toxicology, College of Public Health and Health Professions, University of Florida, Gainesville, FL, USA

6. f Department of Biological and Environmental Sciences, Georgia College and State University, Campus Box 81, Milledgeville, GA 31061, USA

Abstract

ABSTRACT The World Health Organization classifies leptospirosis as a significant public health concern, predominantly affecting impoverished and unsanitary regions. By using the Pensacola Bay System as a case study, this study examines the underappreciated susceptibility of developed subtropical coastal ecosystems such as the Pensacola Bay System to neglected zoonotic pathogens such as Leptospira. We analyzed 132 water samples collected over 12 months from 44 distinct locations with high levels of Escherichia coli (>410 most probable number/100 mL). Fecal indicator bacteria (FIB) concentrations were assessed using IDEXX Colilert-18 and Enterolert-18, and an analysis of water physiochemical characteristics and rainfall intensity was conducted. The LipL32 gene was used as a quantitative polymerase chain reaction (qPCR) indicator to identify the distribution of Leptospira interrogans. The results revealed 12 instances of the presence of L. interrogans at sites with high FIB over various land cover and aquatic ecosystem types. Independent of specific rainfall events, a seasonal relationship between precipitation and elevated rates of fecal bacteria and leptospirosis was found. These findings highlight qPCR's utility in identifying pathogens in aquatic environments and the widespread conditions where it can be found in natural and developed areas.

Funder

U.S. Environmental Protection Agency

Publisher

IWA Publishing

Link

https://iwaponline.com/jwh/article-pdf/22/5/923/1426444/jwh0220923.pdf

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