SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene-Reference-Cited by-同舟云学术

SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene

Published:2020-10-01 Issue:39 Volume:25 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Ziegler Katharina¹²,Steininger Philipp²,Ziegler Renate¹,Steinmann Jörg¹,Korn Klaus²,Ensser Armin²^ORCID

Affiliation:

1. Institute of Clinical Hygiene, Medical Microbiology and Infectiology, Paracelsus Medical University, Nuremberg, Germany

2. Institute of Virology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nuremberg (FAU) Erlangen, Germany

Abstract

We found that a single nucleotide polymorphism (SNP) in the nucleoprotein gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a patient interfered with detection in a widely used commercial assay. Some 0.2% of the isolates in the EpiCoV database contain this SNP. Although SARS-CoV-2 was still detected by the other probe in the assay, this underlines the necessity of targeting two independent essential regions of a pathogen for reliable detection.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

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