Evaluation of the sensitivity of a measles diagnostic real-time RT-PCR assay incorporating recently observed priming mismatch variants, 2024-Reference-Cited by-同舟云学术

Evaluation of the sensitivity of a measles diagnostic real-time RT-PCR assay incorporating recently observed priming mismatch variants, 2024

Published:2024-07-11 Issue:28 Volume:29 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Beck Andrew S¹^ORCID,Lopareva Elena N¹^ORCID,Hwang Hyun²^ORCID,Hart Derek³^ORCID,de Almeida Marcos⁴^ORCID,Anderson Raydel¹^ORCID,Rota Paul A¹^ORCID,Bankamp Bettina¹^ORCID

Affiliation:

1. Viral Vaccine Preventable Diseases Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, United States

2. Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, United States

3. ASRT Inc, Atlanta, United States

4. Strategic Innovative Solutions Inc., Clearwater, United States

Abstract

We investigated a variant of measles virus that encodes three mismatches to the reverse priming site for a widely used diagnostic real-time RT-PCR assay; reduction of sensitivity was hypothesised. We examined performance of the assay in context of the variant using in silico data, synthetic RNA templates and clinical specimens. Sensitivity was reduced observed at low copy numbers for templates encoding the variant sequence. We designed and tested an alternate priming strategy, rescuing the sensitivity of the assay.

Publisher

European Centre for Disease Control and Prevention (ECDC)

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