Supplementing SARS-CoV-2 genomic surveillance with PCR-based variant detection for real-time actionable information, the Netherlands, June to July 2021-Reference-Cited by-同舟云学术

Supplementing SARS-CoV-2 genomic surveillance with PCR-based variant detection for real-time actionable information, the Netherlands, June to July 2021

Published:2021-10-07 Issue:40 Volume:26 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Molenkamp Richard¹,Fanoy Ewout²,Derickx Leonie³,de Groot Theun⁴,Jonges Marcel⁵,Leenstra Tjalling⁶,Nijhuis Roel⁷,Pas Suzan⁸,Vahidnia Ali⁹,von Wintersdorff Christian¹⁰,Mulder Bert⁴,Koopmans Marion¹

Affiliation:

1. Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands

2. Public Health Service Rotterdam-Rijnmond, Rotterdam, the Netherlands

3. Public Health Service Gelderland-Zuid, Nijmegen, the Netherlands

4. Department of Clinical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, the Netherlands

5. Department of Medical Microbiology and Infection Prevention, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands

6. Department of Infectious Diseases, Public Health Service of Amsterdam, Amsterdam, the Netherlands

7. Laboratory for Medical Microbiology and Medical Immunology, Meander Medical Center, Amersfoort, the Netherlands

8. Microvida Laboratory for Medical Microbiology, Amphia Hospital, Breda, the Netherlands

9. Testing Agency, Ministry of Health, Welfare and Sport, Zeist, the Netherlands

10. Department of Medical Microbiology, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Centre (MUMC+), Maastricht, the Netherlands

Abstract

We evaluated routine testing with SARS-CoV-2 Delta variant-specific RT-PCR in regional hospital laboratories in addition to centralised national genomic surveillance in the Netherlands during June and July 2021. The increase of the Delta variant detected by RT-PCR correlated well with data from genomic surveillance and was available ca 2 weeks earlier. This rapid identification of the relative abundance and increase of SARS-CoV-2 variants of concern may have important benefits for implementation of local public health measures.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

Reference10 articles.

1. A simple RT-PCR melting temperature assay to rapidly screen for widely circulating SARS-CoV-2 variants.;Banada;medRxiv,2021

2. Rapid screening method for the detection of SARS-CoV-2 variants of concern.;Ong;J Clin Virol,2021

3. SARS-CoV-2 variants, spike mutations and immune escape.;Harvey;Nat Rev Microbiol,2021

4. World Health Organization (WHO). Tracking SARS-CoV-2 variants. Geneva: WHO. [Accessed: 06 Oct 2021]. Available from: https://www.who.int/en/activities/tracking-SARS-CoV-2-variants

5. The next phase of SARS-CoV-2 surveillance: real-time molecular epidemiology.;Oude Munnink;Nat Med,2021

Cited by 5 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A straightforward one-step strategy for SARS-CoV-2 diagnosis and screening of variants of concern: a multicentre study;Memórias do Instituto Oswaldo Cruz;2023

2. Case report: BA.1 subvariant showing a BA.2-like pattern using a variant-specific PCR assay due to a single point mutation downstream the spike 69/70 deletion;Virology Journal;2022-10-27

3. Quantitative Reverse Transcription PCR Surveillance of SARS-CoV-2 Variants of Concern in Wastewater of Two Counties in Texas, United States;ACS ES&T Water;2022-07-06

4. Global landscape of SARS-CoV-2 genomic surveillance and data sharing;Nature Genetics;2022-03-28

5. It did not stop there: rapid substitution of circulating SARS-CoV-2 variant of concern B.1.1.7 (Alpha) by variant of concern B.1.617.2 (Delta) and further evolution of different Delta sublineages in Southern Saxony-Anhalt in late summer 2021;LaboratoriumsMedizin;2021-12-14