New insights into the activity of Pseudomonas aeruginosa cd1 nitrite reductase

Author:

Rinaldo Serena1,Arcovito Alessandro2,Giardina Giorgio1,Castiglione Nicoletta1,Brunori Maurizio1,Cutruzzolà Francesca1

Affiliation:

1. Dipartimento di Scienze Biochimiche “A. Rossi Fanelli” and Istituto di Biologia e Patologia Molecolari del CNR

2. Sapienza, Università di Roma, Rome, Italy

Abstract

The cytochrome cd1 nitrite reductases are enzymes that catalyse the reduction of nitrite to nitric oxide (NO) in the bacterial energy conversion denitrification process. These enzymes contain two different redox centres: one covalently bound c-haem, which is reduced by external donors, and one peculiar d1-haem, where catalysis occurs. In the present paper, we summarize the current understanding of the reaction of nitrite reduction in the light of the most recent results on the enzyme from Pseudomonas aeruginosa and discuss the differences between enzymes from different organisms. We have evidence that release of NO from the ferrous d1-haem occurs rapidly enough to be fully compatible with the turnover, in contrast with previous hypotheses, and that the substrate nitrite is able to displace NO from the d1-haem iron. These results shed light on the mechanistic details of the activity of cd1 nitrite reductases and on the biological role of the d1-haem, whose presence in this class of enzymes has to date been unexplained.

Publisher

Portland Press Ltd.

Subject

Biochemistry

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