A simple method for purification of rat hepatic microsomal cytochrome b5-Reference-Cited by-同舟云学术

A simple method for purification of rat hepatic microsomal cytochrome b5

Published:1985-02-15 Issue:1 Volume:226 Page:339-341
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Burchell A

Abstract

Rat hepatic microsomal cytochrome b5 was purified to homogeneity by solubilization with the detergent Lubrol 12-A9 and chromatography on Fractogel TSK DEAE-650(S). The protein was obtained in high yield (52-87%) in 8 h, and only one polypeptide band, of Mr 16 600, was visible after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/226/1/339/583149/bj2260339.pdf

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