Meta-analysis based gene expression profiling reveals functional genes in ovarian cancer-Reference-Cited by-同舟云学术

Meta-analysis based gene expression profiling reveals functional genes in ovarian cancer

Published:2020-11 Issue:11 Volume:40 Page:
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Zhao Lin¹,Li Yuhui²,Zhang Zhen¹,Zou Jing³,Li Jianfu⁴,Wei Ran¹,Guo Qiang¹,Zhu Xiaoxiao¹,Chu Chu¹,Fu Xiaoxiao¹,Yue Jinbo⁵,Li Xia¹^ORCID

Affiliation:

1. Department of Oncology, Institute of Basic Medicine, The First Affiliated Hospital of Shandong First Medical University, No. 18877 Jingshi Road, Jinan 250062, China

2. Department of Outpatient, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, No. 440, Ji Yan Road, Jinan 250117, China

3. Affiliated Hospital of Shandong University of Traditional Chinese Medicine, No. 42 Wenhua Xi Road, Jinan 250011, China

4. Surgical Planning Laboratory, Department of Oral and Maxillofacial Surgery, Houston Methodist Research Institute, Houston, TX, U.S.A.

5. Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, No. 440, Ji Yan Road, Jinan 250117, China

Abstract

Abstract Background: Ovarian cancer causes high mortality rate worldwide, and despite numerous attempts, the outcome for patients with ovarian cancer are still not well improved. Microarray-based gene expressional analysis provides with valuable information for discriminating functional genes in ovarian cancer development and progression. However, due to the differences in experimental design, the results varied significantly across individual datasets. Methods: In the present study, the data of gene expression in ovarian cancer were downloaded from Gene Expression Omnibus (GEO) and 16 studies were included. A meta-analysis based gene expression analysis was performed to identify differentially expressed genes (DEGs). The most differentially expressed genes in our meta-analysis were selected for gene expression and gene function validation. Results: A total of 972 DEGs with P-value < 0.001 were identified in ovarian cancer, including 541 up-regulated genes and 431 down-regulated genes, among which 92 additional DEGs were found as gained DEGs. Top five up- and down-regulated genes were selected for the validation of gene expression profiling. Among these genes, up-regulated CD24 molecule (CD24), SRY (sex determining region Y)-box transcription factor 17 (SOX17), WFDC2, epithelial cell adhesion molecule (EPCAM), innate immunity activator (INAVA), and down-regulated aldehyde oxidase 1 (AOX1) were revealed to be with consistent expressional patterns in clinical patient samples of ovarian cancer. Gene functional analysis demonstrated that up-regulated WFDC2 and INAVA promoted ovarian cancer cell migration, WFDC2 enhanced cell proliferation, while down-regulated AOX1 was functional in inducing cell apoptosis of ovarian cancer. Conclusion: Our study shed light on the molecular mechanisms underlying the development of ovarian cancer, and facilitated the understanding of novel diagnostic and therapeutic targets in ovarian cancer.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/doi/10.1042/BSR20202911/897667/bsr-2020-2911.pdf

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