Chilling or chemical induction of dormancy release in blackcurrant (Ribes nigrum) buds is associated with characteristic shifts in metabolite profiles

Author:

Hancock Robert D.1ORCID,Schulz Elisa2,Verrall Susan R.3,Taylor June4,Méret Michaël2,Brennan Rex M.1,Bishop Gerard J.5ORCID,Else Mark4,Cross Jerry V.4,Simkin Andrew J.6ORCID

Affiliation:

1. 1Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, U.K.

2. 2MetaSysX GmbH, Am Mühlenberg 11, 14476 Potsdam-Golm, Germany

3. 3Ecological Sciences, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, U.K.

4. 4NIAB, New Road, East Malling, Kent ME19 6BJ, U.K.

5. 5NIAB, Huntingdon Road, Cambridge CB3 0LE, U.K.

6. 6School of Life Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, U.K.

Abstract

This study reveals striking differences in the content and composition of hydrophilic and lipophilic compounds in blackcurrant buds (Ribes nigrum L., cv. Ben Klibreck) resulting from winter chill or chemical dormancy release following treatment with ERGER, a biostimulant used to promote uniform bud break. Buds exposed to high winter chill exhibited widespread shifts in metabolite profiles relative to buds that experience winter chill by growth under plastic. Specifically, extensive chilling resulted in significant reductions in storage lipids and phospholipids, and increases in galactolipids relative to buds that experienced lower chill. Similarly, buds exposed to greater chill exhibited higher levels of many amino acids and dipeptides, and nucleotides and nucleotide phosphates than those exposed to lower chilling hours. Low chill buds (IN) subjected to ERGER treatment exhibited shifts in metabolite profiles similar to those resembling high chill buds that were evident as soon as 3 days after treatment. We hypothesise that chilling induces a metabolic shift which primes bud outgrowth by mobilising lipophilic energy reserves, enhancing phosphate availability by switching from membrane phospholipids to galactolipids and enhancing the availability of free amino acids for de novo protein synthesis by increasing protein turnover. Our results additionally suggest that ERGER acts at least in part by priming metabolism for bud outgrowth. Finally, the metabolic differences presented highlight the potential for developing biochemical markers for dormancy status providing an alternative to time-consuming forcing experiments.

Funder

UKRI | Biotechnology and Biological Sciences Research Council

Publisher

Portland Press Ltd.

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