In vitro and in vivo biofilm adhesion to esthetic coated arch wires and its correlation with surface roughness

Abstract

ABSTRACT Objective:  To evaluate the in vitro ability of esthetic coated rectangular arch wires to retain oral biofilms and in vivo biofilm formation on these wires after 4 and 8 weeks of clinical use and to correlate the findings with the surface roughness of these wires. Materials and Methods:  Three brands of esthetic coated nickel-titanium (NiTi) arch wires were selected. Arch wires retrieved after 4 and 8 weeks of intraoral use were obtained from 30 orthodontic patients. Surface roughness (SR) was assessed with an atomic force microscope. In vitro adhesion assays were performed using Streptococcus mutans (MS), Staphylococcus aureus, and Candida albicans. The amount of bacterial adhesion was quantified using the colony-count method. Paired t-test, analysis of variance, post hoc Tukey's test, and Pearson's correlation coefficient test were used for statistical analysis at the .05 level of significance. Results:  In vitro bacterial adhesion showed significant differences between wires in terms of MS adhesion (P  =  .01). All wires showed significant increases in SR (P  =  .001 after 4 weeks and .007 after 8 weeks) and biofilm adhesion (P  =  .0001 after 4 weeks and .045 after 8 weeks) after intraoral exposure. A significant positive correlation (P  =  .001 after 4 weeks and .05 after 8 weeks) was observed between these two variables in vivo, but the correlation was not significant for in vitro bacterial adhesion. Conclusions:  SR and biofilm adhesion increased after intraoral use at all time intervals. There was a positive correlation between SR and biofilm adhesion in vivo only.