Abstract
SUMMARY
The uptake and retention of [3H]oestradiol-17β by human uterine and muscle tissue was studied in vitro by incubation of tissue slices. The binding characteristics of immature rat and adult human tissue were compared. Human endometrium had a greater affinity for tritiated oestradiol than human myometrium. Human uterine tissues bound less [3H]oestradiol-17β than rat uterine horns. The difference in retention between a target and non-target tissue in the human tissue was much smaller than in the rat, although still highly significant. Binding was inhibited by low concentrations of oestradiol-17β, U-11100A and hexoestrol, but was not inhibited by the same concentrations of oestrone, oestriol or oestradiol-17α. Freezing the uterine tissue resulted in a loss of specific binding activity. Binding was shown to vary with different uterine specimens. There was a significant difference in [3H]oestradiol-17β retention between the proliferative and secretory phases of the menstrual cycle. The highest endometrial retention was found in endometrial carcinoma.
Subject
Endocrinology,Endocrinology, Diabetes and Metabolism
Cited by
41 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献