Author:
BURKE C. W.,SHAKESPEAR R. A.
Abstract
SUMMARY
Thyroxine (T4) and tri-iodothyronine (T3) were coupled to human serum albumin (HSA) with carbodi-imide. By adsorption chromatography on Sephadex G-25, fractions containing purified conjugate, but not reversibly-bound T3 or T4, were obtained, and this procedure took 5 h; considerably less than the conventional dialysis technique. Highly specific high-titre antisera were produced in rabbits and guinea-pigs by injection of these fractions in Freund's adjuvant.
Subject
Endocrinology,Endocrinology, Diabetes and Metabolism
Cited by
15 articles.
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