Determination of Pneumococcal Serotypes by Sequetyping and Sequential Conventional Multiplex PCR in the Vaccine Era
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Published:2023-10-31
Issue:4
Volume:46
Page:1233-1244
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ISSN:2231-8542
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Container-title:Pertanika Journal of Tropical Agricultural Science
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language:en
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Short-container-title:JTAS
Author:
Abdul Rahman Nurul Asyikin,Mohd Desa Mohd Nasir,Masri Siti Norbaya,Mohd Taib Niazlin,Sulaiman Nurshahira,Dzaraly Nurul Diana,Hazman Hazmin
Abstract
Pneumococcal serotyping is required for epidemiological surveillance to guide vaccination strategy. DNA-based approaches are more affordable, but the combination of sequetyping and sequential conventional multiplex polymerase chain reaction (cmPCR) may complement one another. A total of 101 isolates were subjected to sequetyping and sequential cmPCR following previously published protocols, and the outputs were compared. The sequetyping method determined up to the serotype level for 99 isolates (98%). On the other hand, the sequential cmPCR technique identified 91 isolates (90.1%), with 63 of them (62.4%) up to the serotype level. Sequetyping generated discrete serotypes for 6A/B, 11A/D, 15A/F, and 15B/C as 6A (n = 11), 6B (n = 10), 11A (n = 5), 15C (n = 1), and 15A (n = 1). In conclusion, the cpsB gene sequetyping method produced a comparable output with sequential cmPCR, further discriminating some sub-serogroups among the isolate collection.
Publisher
Universiti Putra Malaysia
Subject
Plant Science,Forestry
Reference23 articles.
1. Batt, S. L., Charalambous, B. M., McHugh, T. D., Martin, S., & Gillespie, S. H. (2005). Novel PCR-restriction fragment length polymorphism method for determining serotypes or serogroups of Streptococcus pneumoniae isolates. Journal of Clinical Microbiology, 43(6), 2656–2661. https://doi.org/10.1128/JCM.43.6.2656-2661.2005 2. Centers for Disease Control and Prevention. (2021). PCR-based methods for identification of S. pneumoniae serotypes/serogroups. CDC. https://www.cdc.gov/streplab/pneumococcus/resources.html 3. Daningrat, W. O. D., Amalia, H., Ayu, I. M., Satzke, C., & Safari, D. (2022). Carriage of Streptococcus pneumoniae in children under five years of age prior to pneumococcal vaccine introduction in Southeast Asia: A systematic review and meta-analysis (2001–2019). Journal of Microbiology, Immunology and Infection, 55(1), 6–17. https://doi.org/10.1016/j.jmii.2021.08.002 4. Dzaraly, N. D., Mohd Desa, M. N., Muthanna, A. R., Masri, S. N., Taib, N. M., Suhaili, Z., Sulaiman, N., Baharin, N. H. Z., Shuan, C. Y., Ariffin, Z., Rahman, N. I. A., Mohd Rani, F., Palanisamy, N. K., Soh, T. S. T., & Abdullah, F. H. (2021). Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia. Scientific Reports, 11, 8220. https://doi.org/10.1038/s41598-021-87428-z 5. Ganaie, F., Maruhn, K., Li, C., Porambo, R. J., Elverdal, P. L., Abeygunwardana, C., van der Linden, M., Duus, J. Ø., Sheppard, C. L., & Nahm, M. H. (2021). Structural, genetic, and serological elucidation of Streptococcus pneumoniae serogroup 24 serotypes: Discovery of a new serotype, 24C, with a variable capsule structure. Journal of Clinical Microbiology, 59(7), e00540-21. https://doi.org/10.1128/JCM.00540-21
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