The inositol 5-phosphatase INPP5K participates in the fine control of ER organization

Author:

Dong Rui1234ORCID,Zhu Ting56,Benedetti Lorena1234,Gowrishankar Swetha1234,Deng Huichao56ORCID,Cai Yiying1234,Wang Xiangming5,Shen Kang5678,De Camilli Pietro12349ORCID

Affiliation:

1. Department of Neuroscience, Yale University School of Medicine, New Haven, CT

2. Department of Cell Biology, Yale University School of Medicine, New Haven, CT

3. Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT

4. Program in Cellular Neuroscience, Neurodegeneration and Repair, New Haven, CT

5. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China

6. University of Chinese Academy of Sciences, Beijing, China

7. Department of Biology, Stanford University School of Medicine, Stanford, CA

8. Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA

9. Kavli Institute for Neurosciences, Yale University School of Medicine, New Haven, CT

Abstract

INPP5K (SKIP) is an inositol 5-phosphatase that localizes in part to the endoplasmic reticulum (ER). We show that recruitment of INPP5K to the ER is mediated by ARL6IP1, which shares features of ER-shaping proteins. Like ARL6IP1, INPP5K is preferentially localized in ER tubules and enriched, relative to other ER resident proteins (Sec61β, VAPB, and Sac1), in newly formed tubules that grow along microtubule tracks. Depletion of either INPP5K or ARL6IP1 results in the increase of ER sheets. In a convergent but independent study, a screen for mutations affecting the distribution of the ER network in dendrites of the PVD neurons of Caenorhabditis elegans led to the isolation of mutants in CIL-1, which encodes the INPP5K worm orthologue. The mutant phenotype was rescued by expression of wild type, but not of catalytically inactive CIL-1. Our results reveal an unexpected role of an ER localized polyphosphoinositide phosphatase in the fine control of ER network organization.

Funder

National Institutes of Health

Howard Hughes Medical Institute

China Scholarship Council

BrightFocus Foundation

National Natural Science Foundation of China

Chinese Academy of Sciences

State Administration of Foreign Experts Affairs

Youth Innovation Promotion Association of the Chinese Academy of Sciences

Publisher

Rockefeller University Press

Subject

Cell Biology

Reference42 articles.

1. The CIL-1 PI 5-phosphatase localizes TRP Polycystins to cilia and activates sperm in C. elegans;Bae;Curr. Biol.,2009

2. Phosphoinositides: tiny lipids with giant impact on cell regulation;Balla;Physiol. Rev.,2013

3. Visualization of cellular phosphoinositide pools with GFP-fused protein-domains;Balla;Curr. Protoc. Cell Biol.,2009

4. Hereditary spastic paraplegias: membrane traffic and the motor pathway;Blackstone;Nat. Rev. Neurosci.,2011

5. A conserved amphipathic helix is required for membrane tubule formation by Yop1p;Brady;Proc. Natl. Acad. Sci. USA.,2015

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3