Evaluation of Diuretic Activity of Siddha Poly Herbal Formulation <i>Vithu Vagai Chooranam</i> in Rodents

Abstract

The current pharmacological investigation was performed to study the diuretic activity of Siddha polyherbal formulation Vithu Vagai Chooranam (VVC) in Wistar albino rats using the Lipschitz method. The animals were divided into four Groups (I, II, III, and IV) of six rats (n = 6) each. Group I served as control treated with milk (1ml/kg p.o), and Group II received Standard furosemide (10 mg/kg, p.o). Groups III and Group IV were treated with test drug at a dose of 70mg/kg and 90mg/kg, p.o, respectively. The urine volume, pH, and electrolyte concentration (Na+, K+ and Cl-) were estimated at the end of 24 hours. Diuretic index, Lipschitz values, and natriuretic index were also calculated from the results to make a comparison with control and furosemide-treated groups. Statistical analysis was carried out using a one-way analysis of variance, followed by Dunnett’s ‘t’ test. Oral administration of VVC at both doses significantly increased the urine output and concentration of urinary electrolytes (p < 0.01). The VVC-induced diuresis pattern was almost like that of frusemide. The findings revealed that the Vithu vagai chooranam possessed significant diuretic activity in experimental rats.