Identification and Quantification of Kaempferol From Ethyl Acetate Fraction of <i>Pluchea wallichiana</i> DC Leaves

Abstract

P. wallichiana DC, commonly known as camphor weed, is practically used in treatment of inflammatory disorders. Identification of kaempferol was done using High-Resolution Liquid Chromatography-Mass Spectrometry (HRLC-MS). High-Performance Thin-Layer Chromatography (HPTLC) method was performed and validated for the quantification of kaempferol. With a correlation coefficient of 0.997, the linearity range for kaempferol was 200 to 700 ng/spot. The suggested method had a recovery rate between 99.60 and 99.85 %. Limit of detection and Limit of quantification were determined to be 25 and 76.47 ng per spot, respectively. The amount of kaempferol was found to be 7.2% in ethyl acetate fraction of leaves. This is the first report of identification and quantification of kaempferol from P. wallichiana DC through HRLC-MS and HPTLC methods.