Determination of Lethal Dose of Disodium 5’ Ribonucleotide (E635) on Embryonic Development of <i>Gallus gallus</i>

Abstract

Disodium 5’ Ribonucleotide (E635), a food additive, has FDA safety, but concerns about its potential toxicity in developmental biology have been raised due to limited research on its lethal effects. The present research aimed to investigate the lethal dose of E635 when induced into a vertebrate chick embryo model. Pilot doses of E635 ranging from 1 μg- 400 μg per egg were inoculated. Consequently, fertilized chick eggs were randomly assigned to 6 groups, including 1 control and 5 differently intoxicated groups of E635 doses per egg. E635 was induced in eggs by in vivo administration and incubated for up to 15 days. LD50 was determined by using probit analysis in SPSS. The study showed morphological, physiological and survival alterations. A dose-dependent mortality was observed by E635 induction in the present study. The results were interpreted for functional and biological changes in the developing embryo of Gallus gallus to confirm the induced toxicity. LD50 of E635 on chick embryo was found to be 0.054 μg per egg.