Affiliation:
1. Federal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences; Orenburg State University
2. Federal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences
Abstract
In this paper, the authors evaluated the metabolic parameters of the scar content as a result of the transformation of a vegetable substrate (linseed cake) in the design of a bioreactor (continuous fermenter).Methods. The study was carried out in vitro using a specialized technique using a bioreactor, the duration of fermentation in which was 20 days of continuous fermentation without additional addition of a substrate. The scar content was obtained from Kazakh white-headed bull calves aged 15 months with chronic scar fistula. The level of volatile fatty acids in the contents of the rumen was determined by gas chromatography. The chemical composition of the test substrate was determined according to generally accepted methods.Results. Continuous fermentation of linseed cake in a bioreactor for 14 days showed the ability of this substrate to maintain the activity of the scar microbiota for the decomposition of nutritional components. As a result of the splitting of flaxseed cake, the preservation of a sufficiently high level of volatile fatty acids and nitrogen metabolites in the rumen inoculum was noted, as well as an increase in the digestibility of DM, CF, CP in this feed medium, was noted. The results of this study show that flaxseed cake can be used for the synthesis of microbial protein and as a source of protein and fat when added to the diet of ruminants using a continuous cultivation system.