Affiliation:
1. Federal Scientific Center — All-Russian Scientific Research Institute of Experimental Veterinary named after K.I. Scriabin and Y.R. Kovalenko of the Russian Academy of Sciences
Abstract
Reproduction of a highly productive herd of cattle with genetic resistance to leukemia is one of the most important tasks of livestock breeding, achievable by an integrated approach, including genetic and selection research, including those aimed at studying the associative relationship between the iNOS Bos taurus gene polymorphism and breeding value, and also with the susceptibility and resistance of animals to this disease. The objectives of the study were to map the identified polymorphic restriction sites in 6 SNP markers (AH13-1, AH13-2, AH13-3, AH13-4, AH13-5 and AH13-6) of the iNOS Bos taurus gene and program calculation of PCR-PDRF profiles of possible genotypes, followed by testing of the developed method of cattle genotyping cattle by the listed markers. The theoretical and analytical part of the conducted bioinformatics study expanded knowledge about the polymorphic restriction sites of the 6 mentioned SNP markers and the corresponding PCR-RFLP profiles of their possible genotypes, including complex genotypes of the polymorphic markers AH13-1 and AH13-6 generated during restriction mapping the analyzed DNA sequence, limited by primers iNOS-F and iNOS-R. Further, the theoretical possibility of detecting the listed markers by analyzing the length polymorphism ofrestriction fragments of amplified DNA was supported by experimental data obtained as a result of testing the developed method for PCR-RFLP genotyping of cattle using the listed markers with 5 selected restriction endonucleases and isoschizomers (HinfI, AspS9I, HpyAV, Sse9I and Bst4CI) predominantly Russian-made, which ultimately had a positive impact on the cost of the research conducted.