Simple flow cytometry method using a myeloma panel that easily reveals clonal proliferation of mature B-cells

Author:

Araki Mika1,Mitsuhashi Takayuki2ORCID,Yatabe Yoko1,Arai Tomoko1,Yokota Hiromitsu1,Okita Hajime3,Sakurai Masatoshi4,Tsukada Nobuhiro5,Kataoka Keisuke46,Matsushita Hiromichi2ORCID

Affiliation:

1. Clinical Laboratory , Keio University Hospital , Tokyo , Japan

2. Department of Laboratory Medicine , Keio University School of Medicine , Tokyo , Japan

3. Department of Diagnostic Pathology , Keio University School of Medicine , Tokyo , Japan

4. Division of Hematology, Department of Medicine , Keio University School of Medicine , Tokyo , Japan

5. Department of Hematology , Japanese Red Cross Medical Center , Tokyo , Japan

6. Division of Molecular Oncology , National Cancer Center Research Institute , Tokyo , Japan

Publisher

Walter de Gruyter GmbH

Reference10 articles.

1. Flores-Montero, J, de Tute, R, Paiva, B, Perez, JJ, Böttcher, S, Wind, H, et al.. Immunophenotype of normal vs. myeloma plasma cells: toward antibody panel specifications for MRD detection in multiple myeloma. Cytometry B Clin Cytom 2016;90:61–72. https://doi.org/10.1002/cyto.b.21265.

2. Rosado, FG, Morice, WG, He, R, Howard, MT, Timm, M, McPhail, ED. Immunophenotypic features by multiparameter flow cytometry can help distinguish low grade B-cell lymphomas with plasmacytic differentiation from plasma cell proliferative disorders with an unrelated clonal B-cell process. Br J Haematol 2015;169:368–76. https://doi.org/10.1111/bjh.13303.

3. Qiu, L, Nwogbo, OV, Medeiros, LJ, Thakral, B, Li, S, Xu, J, et al.. Lymphoplasmacytic lymphoma with IgG or IgA paraprotein: a study of 29 cases including cases that can mimic plasma cell neoplasms. Hum Pathol 2022;130:47–57. https://doi.org/10.1016/j.humpath.2022.10.005.

4. Brozic, A, Marinsek, ZP, Bucek, S, Cemazar, M, Prevodnik, VK. Usefulness of Bcl-2 expression and the expression of cytoplasmic immunoglobulin light chains in the differentiation between B-cell lymphoma and reactive lymphocytic proliferations in FNA. Int J Mol Sci 2019;20:2648. https://doi.org/10.3390/ijms20112648.

5. Matsushita, H, Nakamura, N, Tanaka, Y, Ohgiya, D, Tanaka, Y, Damdinsuren, A, et al.. Clinical and pathological features of B-cell non-Hodgkin lymphomas lacking the surface expression of immunoglobulin light chains. Clin Chem Lab Med 2012;50:1665–70. https://doi.org/10.1515/cclm-2011-1854.

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