Use of dried blood spots for monitoring inflammatory and nutritional biomarkers in the elderly
Author:
Vialaret Jérôme1ORCID, Vignon Margaux1ORCID, Hirtz Christophe1ORCID, Badiou Stéphanie2, Baptista Gregory3, Fichter Laura1, Dupuy Anne-Marie2ORCID, Maceski Aleksandra Maleska1, Fayolle Martin12, Brousse Mehdi12, Cristol Jean-Paul2, Jeandel Claude3, Lehmann Sylvain1ORCID
Affiliation:
1. LBPC-PPC, Univ Montpellier, CHU Montpellier, INM INSERM , Montpellier , France 2. Department of Biochemistry and Hormonology , Univ Montpellier, CHU Montpellier, INSERM , Montpellier , France 3. Centre de gérontologie clinique Antonin-Balmès, Univ Montpellier, CHU Montpellier , Montpellier , France
Abstract
Abstract
Objectives
Blood microsampling, particularly dried blood spots (DBSs), is an attractive minimally-invasive approach that is well suited for home sampling and predictive medicine associated with longitudinal follow-up of the elderly. However, in vitro diagnostic quantification of biomarkers from DBS poses a major challenge. Clinical mass spectrometry can reliably quantify blood proteins in various research projects. Our goal here was to use mass spectrometry of DBS in a real-world clinical setting and compared it to the standard immunoassay method. We also sought to correlate DBS mass spectrometry measurements with clinical indices.
Methods
A clinical trial of diagnostic equivalence was conducted to compare conventional venous samples quantified by immunoassay and DBSs quantified by mass spectrometry in an elderly population. We assayed three protein biomarkers of nutritional and inflammatory status: prealbumin (transthyretin), C-reactive protein, and transferrin.
Results
The analysis of DBSs showed satisfactory variability and low detection limits. Statistical analysis confirmed that the two methods give comparable results at clinical levels of accuracy. In conclusion, we demonstrated, in a real-life setting, that DBSs can be used to measure prealbumin, CRP and transferrin, which are commonly used markers of nutritional status and inflammation in the elderly. However, there was no correlation with patient frailty for these proteins.
Conclusions
Early detection and regular monitoring of nutritional and inflammatory problems using DBS appear to be clinically feasible. This could help resolve major public health challenges in the elderly for whom frailty leads to serious risks of health complications.
Funder
Agence Nationale de la Recherche
Publisher
Walter de Gruyter GmbH
Subject
Biochemistry (medical),Clinical Biochemistry,General Medicine
Reference55 articles.
1. Allison, R, Assadzandi, S, Adelman, M. Frailty: evaluation and management. Am Fam Physician 2021;103:219–26. 2. Arevalo-Rodriguez, I, Smailagic, N, Roqué-Figuls, M, Ciapponi, A, Sanchez-Perez, E, Giannakou, A, et al.. Mini-Mental State Examination (MMSE) for the early detection of dementia in people with mild cognitive impairment (MCI). Cochrane Database Syst Rev 2021;7:CD010783. https://doi.org/10.1002/14651858.cd010783.pub3. 3. Omran, ML, Morley, JE. Assessment of protein energy malnutrition in older persons, Part II: laboratory evaluation. Nutr Burbank Los Angel Cty Calif 2000;16:131–40. https://doi.org/10.1016/s0899-9007(99)00251-8. 4. Trevisan, C, Crippa, A, Ek, S, Welmer, AK, Sergi, G, Maggi, S, et al.. Nutritional status, body mass index, and the risk of falls in community-dwelling older adults: a systematic review and meta-analysis. J Am Med Dir Assoc 2019;20:569–82.e7. https://doi.org/10.1016/j.jamda.2018.10.027. 5. Sullivan, DH, Patch, GA, Walls, RC, Lipschitz, DA. Impact of nutrition status on morbidity and mortality in a select population of geriatric rehabilitation patients. Am J Clin Nutr 1990;51:749–58. https://doi.org/10.1093/ajcn/51.5.749.
|
|