Functionalized gold nanoparticles coated with bacterial alginate and their antibacterial and anticancer activities
Author:
Sindi Hebah A.1ORCID, Hamouda Ragaa A.23ORCID, Alhazmi Nuha M.1ORCID, Abdel-Hamid Marwa S.3ORCID
Affiliation:
1. Department of Biological Sciences, College of Science, University of Jeddah , Jeddah 21589 , Saudi Arabia 2. Department of Biology, Collage of Sciences and Arts Khulais, University of Jeddah , Jeddah 21959 , Saudi Arabia 3. Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City , Sadat City 32897 , Egypt
Abstract
Abstract
Gold nanoparticles (Au-NPs) have several uses for nanobiotechnologists because of their beneficial biomedical properties. Alginates have various biomedical and industrial applications. The aim of this study is to extract alginate from Azotobacter chroococcum, synthesize chemical Au-NPs (Ch/Au-NPs), and load the NPs with the extracted alginate to form Azotobacter alginate gold nanocomposites (Azto/Alg-Au-NCMs). The Ch/Au-NPs and Azto/Alg-Au-NCMs were characterized by UV-spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, energy-dispersive spectroscopy (EDS), X-ray diffraction (XRD), thermogravimetric analysis (TGA), zeta potential, and transmission electron microscopy (TEM). The anticancer activities were determined using the breast cancer cell line MCF-7, human lung cancer cell line H1299, and Vero cell line. The results obtained by UV-spectroscopy exhibited a surface plasmon resonance that was clearly noticeable at 530 nm, and the EDS analysis proved that gold was present in percentages of 50.11 and 28.08 in the Ch/Au-NPs and Azto/Alg-Au-NCMs, respectively. There were several similarities between the alginic acid and the alginate extracted from A. chroococcum, and small modifications were proved by FT-IR spectroscopy. Negative charges were shown by the zeta potential. Crystalline and cubic NPs were shown by XRD analysis and TEM. TGA demonstrated the purity of the Ch/Au-NPs and the existence of organic compounds in the Azto/Alg-Au-NCMs. Both the Ch/Au-NPs and Azto/Alg-Au-NCMs had antibacterial activities against Staphylococcus aureus ATCC 25923, Proteus mirabilis, Enterobacter sp., and Pseudomonas aeruginosa and possessed anticancer activities against MCF-7 and H1299.
Publisher
Walter de Gruyter GmbH
Subject
Health, Toxicology and Mutagenesis,Industrial and Manufacturing Engineering,Fuel Technology,Renewable Energy, Sustainability and the Environment,General Chemical Engineering,Environmental Chemistry
Reference99 articles.
1. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: Cancer J Clin. 2021;71(3):209–49. 2. Soule HD, Vazquez J, Long A, Albert S, Brennan MA. Human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst. 1973;51(5):1409–16. 3. Molina JR, Yang P, Cassivi SD, Schild SE, Adjei AA. Non-small cell lung cancer: Epidemiology, risk factors, treatment, and survivorship. Mayo Clin Proc. 2008;83(5):584–94. 4. Ljungberg B, Campbell SC, Choi HY, Jacqmin D, Lee JE, Weikert S, et al. The epidemiology of renal cell carcinoma. Eur Urol. 2011;60(4):615–21. 5. Centers for Disease Control and Prevention (CDC). Antibiotic resistance threats in the United States. 2019. Retrieved from. https://www.cdc.gov/drugresistance/pdf/threats-report/2019-ar-threats-report-508.pdf.
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