Chitosan nanoparticles encapsulated into PLA/gelatin fibers for bFGF delivery
Author:
Ghasemzaie Niloofar1, Hadjizadeh Afra1, Niknejad Hassan2
Affiliation:
1. Biomaterials and Tissue Engineering Group , Department of Biomedical Engineering, Amirkabir University of Technology , Tehran 1591634311 , Iran 2. Department of Pharmacology , School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran , Iran
Abstract
Abstract
Electrospinning is a trendy method because of the ease of use and the high surface-to-volume ratio. The mechanical and biological properties of polylactic acid (PLA) make it one of the most enticing polymers. Gelatin and PLA together are thought to enhance cellular behavior and hydrophilicity of scaffolds. Furthermore, chitosan nanoparticles (CNPs) can be incorporated into PLA fibers to achieve controlled growth factor release. This study utilized PLA–gelatin nanofibrous scaffolds in which CNPs were encapsulated within PLA fibers to achieve a controlled release of basic fibroblast growth factor (bFGF). To produce CNPs, a simple ionic gelation reaction was used. The optimal diameter of CNPs was determined by investigating chitosan to tricalciumphosphatesodium (TPP) ratio and TPP concentration. Using a spectrophotometer, we measured the release rate of bFGF from CNPS and scaffolds. Images from a scanning electron microscope (SEM) were used to assess the effect of various concentrations of PLA and gelatin on fiber diameter. The results showed that PLA–gelatin scaffolds could stimulate the release of growth factors and promote cell proliferation. Using a two-jet electrospinning device to produce PLA–gelatin fibers in combination with CNPs incorporated within PLA fibers to release the bFGF growth factor is the novelty of this study.
Publisher
Walter de Gruyter GmbH
Subject
Materials Chemistry,Polymers and Plastics,General Chemical Engineering
Reference33 articles.
1. Luu, Y. K., Kim, K., Hsiao, B. S., Chu, B., Hadjiargyrou, M. Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA–PEG block copolymers. J. Contr. Release 2003, 89, 341–353; https://doi.org/10.1016/s0168-3659(03)00097-x. 2. Zhang, Y., Ouyang, H., Lim, C. T., Ramakrishna, S., Huang, Z. Electrospinning of gelatin fibers and gelatin/PCL composite fibrous scaffolds. J. Biomed. Mater. Res. B Appl. Biomater. 2005, 72, 156–165; https://doi.org/10.1002/jbm.b.30128. 3. Yin, A., Zhang, K., McClure, M. J., Huang, C., Wu, J., Fang, J., Mo, X., Bowlin, G. L., Al‐Deyab, S. S., El‐Newehy, M. Electrospinning collagen/chitosan/poly (L-lactic acid-co-ϵ-caprolactone) to form a vascular graft: mechanical and biological characterization. J. Biomed. Mater. Res., Part A 2013, 101, 1292–1301; https://doi.org/10.1002/jbm.a.34434. 4. Gupta, B., Revagade, N., Hilborn, J. Poly (lactic acid) fiber: an overview. Prog. Polym. Sci. 2007, 32, 455–482; https://doi.org/10.1016/j.progpolymsci.2007.01.005. 5. Badami, A. S., Kreke, M. R., Thompson, M. S., Riffle, J. S., Goldstein, A. S. Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly (lactic acid) substrates. Biomaterials 2006, 27, 596–606; https://doi.org/10.1016/j.biomaterials.2005.05.084.
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