β-Elemene regulates epithelial-mesenchymal transformation and inhibits invasion and metastasis of colorectal cancer cells
Author:
Deng Heng1, Chen Guo2, Zhang Jun3
Affiliation:
1. Department of Proctology , Second Hospital Affiliated Anhui University of Traditional Chinese Medicine , Hefei , China 2. Graduate Department , Anhui University of Chinese Medicine , Hefei , China 3. Chinese Medicine Teaching and Research Section , Anhui University of Chinese Medicine , Hefei , China
Abstract
Abstract
Objectives
To study the inhibitory effect of β-elemene on invasion and metastasis of colorectal cancer cells and its possible mechanism.
Methods
Human colon cancer HCT116 cells were treated with different concentrations of β-elemene. The proliferation inhibition rate of the cells was detected by MTT assay, cell migration rate was detected by scratched assay, and cell invasion rate was evaluated by Transwell cell invasion assay. The expressions of Vimentin, E-cadherin, N-cadherin, and β-catenin were detected by Western blotting. The mRNA expressions of Vimentin, E-cadherin, N-cadherin, and β-catenin were detected by real-time PCR.
Results
Compared with the control group, the expressions of migration rate, invasion rate, scratch healing rate, N-cadherin, and Vimentin protein of HCT116 cells were decreased after β-elemene treatment, while the expression of E-cadherin protein was increased, and the inhibition rate of cell proliferation was increased (p<0.05).
Conclusions
β-Elemene may inhibit cell proliferation and invasion and metastasis by inhibiting EMT signaling pathway in human colon cancer cell line HCT116.
Funder
Research Project of Anhui University of Traditional Chinese Medicine Natural Science Foundation of Anhui Province
Publisher
Walter de Gruyter GmbH
Subject
Complementary and alternative medicine
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