Affiliation:
1. Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Iskenderun Technical University , Hatay , Turkey
2. Department of Biochemistry, Faculty of Medicine, Girne American University , Kyrenia , Cyprus
3. Department of Molecular Biology, Faculty of Science and Arts, Kafkas University , Kars , Turkey
4. Department of Zoology, Faculty of Science and Arts, Kafkas University , Kars , Turkey
Abstract
Abstract
Pesticides, which adversely affect the critical metabolic processes of organisms, disrupt the physiological balance by specifically targeting enzymes and may lead to such consequences that may lead to death. It provides benefits in agricultural activities. The p53 protein antagonizes bcl-2, an anti-apoptotic protein character, and induces apoptosis by causing mitochondrial membrane permeability. This study aims to show the effect of acetamiprid, which is an insecticide from the neonicotinoid class, on bcl-2 and p53 immunoreactivity, which has an important place in the apoptotic mechanism in kidney tissue. A total of four groups including control and three experimental groups (the acetamiprid was administered 5, 10, and 15 mg kg−1) were formed in the study. After acetamiprid was administered via gavage for 14 days, the kidney tissues taken from the mice, which were sacrificed by cervical dislocation, were fixed in 10% formaldehyde solution for histological and immunohistochemical analyses, and as a result of routine tissue follow-up, the sections were blocked in paraffin and stained with haematoxylin–eosin and immunostaining. The histopathological examinations revealed that while the kidney tissue had a normal structure in the control group, degeneration in the distal and proximal tubules, glomerular degeneration, increase in the capsular area, glomerular atrophy, and haemorrhage were determined in the acetamiprid groups at increasing severity and frequency depending on the dose of the applied substance. In the kidney tissue, Bcl-2 and p53 immunoreactivity was observed in glomerular cells, sinusoidal epithelium, and proximal and distal tubule cells. The acetamiprid caused pathological changes in the kidneys in the dose range used. This effect also affects the expression of bcl-2 and p53 genes, which are biomarkers in the apoptotic mechanism. As acetamiprid accumulates in tissues, it increases the expression of p53 from cell death receptors, while suppressing the anti-apoptotic bcl-2 expression.
Reference47 articles.
1. Gill P, Dommalapati S, Rao DS, Gupta RK, Singh D, Dahiya T, et al. Toxicity characteristics of acetamiprid insecticides against adult earthworms, eisenia fetida. Agric Mech Asia. 2021;51(1):1269–76.
2. Nur G, Deveci HA, Koc E. Preservation of Vitamin-E against nephrotoxic effect induced by subacute dichlorvos application. Fresenius Environ Bull. 2021;30(7):8651–9.
3. Hallmann CA, Foppen RP, van Turnhout CA, de Kroon H, Jongejans E. Declines in insectivorous birds are associated with high neonicotinoid concentrations. Nature. 2014;51:341–3. 10.1038/nature13531.
4. Gazzi EN, Sorodoc V, Jaba IM, Lionte C, Bologa C, Lupusoru CE, et al. Profile of adult acute cholinesterase inhibitors substances poisoning - A 30 years analysis. Open Med. 2015;10:278–84. 10.1515/med-2015-0041.
5. Jeschke P, Nauen R, Beck ME. Nicotinic acetylcholine receptor agonists: a milestone for modern crop protection. Angew Chem Int Ed Engl. 2013;52(36):9464–85. 10.1002/anie.201302550.
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