Overexpression of long non-coding RNA ANRIL in B-acute lymphoblastic leukemia
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Published:2021-06-29
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ISSN:2008-8892
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Container-title:Iranian Journal of Pediatric Hematology & Oncology
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language:
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Short-container-title:IJPHO
Author:
Bahraini Mehran,Faranoush Mohammad,Naseri Mobaraki Sepideh,Paridar Mostafa,Amini Ali,Manafi Shabestari Rima,Safa Majid
Abstract
Background: Dysregulation of LncRNA antisense non-coding RNA in the INK4 locus (ANRIL) expression is implicated in pathogenesis and disease progression of a variety of cancer types. However, the expression level of ANRIL in pediatric patients with B-cell precursor acute lymphoblastic leukemia (BCP-ALL) has not been elucidated, yet. The present study is an attempt to evaluate the expression level of ANRIL at different clinical stages in pediatric patients with BCP-ALL.
Materials and Methods: This case-control study was conducted in Tehran, Iran on peripheral blood samples obtained at diagnosis, complete remission, and relapse phases from a total of 50 pediatric BCP-ALL patients who were admitted to Mahak Hospital and Rehabilitation Complex, and Rasul Akram Hospital. The ANRIL expression analysis was performed by the quantitative real-time polymerase chain reaction (qRT-PCR) method. To test the statistical significance, a nonparametric Mann-Whitney U test was used.
Results: The mean fold-changes of ANRIL gene expression in newly diagnosed patients were [31.51 (18.28 to 44.75)] compared to the control group [1.06 (0.73 to 1.38)] indicating significant overexpression (P<0.001). ANRIL fold-changes significantly declined following achievement of complete remission [1.24 (0.80 to 1.69)] compared to the newly diagnosed patients (p<0.001) and increased as the patients experienced relapse [285.4 (269.70 to 301) (P<0.001)].
Conclusions: LncRNA ANRIL may contribute to BCP-ALL pathogenesis and disease progression; casting new light on the application of ANRIL as a potential biomarker or therapeutic target in BCP-ALL.
Subject
Oncology,Hematology,Pediatrics, Perinatology and Child Health
Cited by
2 articles.
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