Cantharidin decreased viable cell number in human osteosarcoma U-2 OS cells through G2/M phase arrest and induction of cell apoptosis

Author:

Chen Chia-Ching1,Chueh Fu-Shin2,Peng Shu-Fen1,Huang Wen-Wen1,Tsai Chang-Hai34,Tsai Fuu-Jen45,Huang Chih-Yang678910,Tang Chih-Hsin8911,Yang Jai-Sing10,Hsu Yuan-Man1,Yin Mei-Chin210,Huang Yi-Ping12,Chung Jing-Gung111

Affiliation:

1. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan

2. Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan

3. China Medical University Children’s Hospital, China Medical University, Taichung, Taiwan

4. Department of Healthcare Administration, Asia University, Taichung, Taiwan

5. School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan

6. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan

7. Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan

8. Chinese Medicine Research Center, China Medical University, Taichung, Taiwan

9. Department of Pharmacology, School of Medicine, China Medical University, Taichung, Taiwan

10. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan

11. Department of Biotechnology, College of Medical and Health Science, Asia University, Taichung, Taiwan

12. Department of Physiology, College of Medicine, China Medical University, Taichung, Taiwan

Abstract

ABSTRACT Cantharidin (CTD), a sesquiterpenoid bioactive substance, has been reported to exhibit anticancer activity against various types of cancer cells. The aim of the present study was to investigate the apoptosis effects and the underlying mechanisms of CTD on osteosarcoma U-2 OS cells. Results showed that CTD induced cell morphologic changes, reduced total viable cells, induced DNA damage, and G2/M phase arrest. CTD increased the production of reactive oxygen species and Ca2+, and elevated the activities of caspase-3 and −9, but decreased the level of mitochondrial membrane potential. Furthermore, CTD increased the ROS- and ER stress-associated protein expressions and increased the levels of pro-apoptosis-associated proteins, but decreased that of anti-apoptosis-associated proteins. Based on these observations, we suggested that CTD decreased cell number through G2/M phase arrest and the induction of cell apoptosis in U-2 OS cells and CTD could be a potential candidate for osteosarcoma treatments.

Funder

China Medical University

Ministry of Science and Technology

Medical Research Core Facilities Center, Office of Research & Development at China Medical University, Taichung, Taiwan

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

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