Abstract
AbstractHuman and other genomes encode tens of thousands of long noncoding RNAs (lncRNAs), the vast majority of which remain uncharacterised. High-throughput functional screening methods, notably those based on pooled CRISPR-Cas perturbations, promise to unlock the biological significance and biomedical potential of lncRNAs. Such screens are based on libraries of single guide RNAs (sgRNAs) whose design is critical for success. Few off-the-shelf libraries are presently available, and lncRNAs tend to have cell-type-specific expression profiles, meaning that library design remains in the hands of researchers. Here we introduce the topic of pooled CRISPR screens for lncRNAs and guide readers through the three key steps of library design: accurate annotation of transcript structures, curation of optimal candidate sets, and design of sgRNAs. This review is a starting point and reference for researchers seeking to design custom CRISPR screening libraries for lncRNAs.
Funder
schweizerischer nationalfonds zur förderung der wissenschaftlichen forschung
medical faculty of the university and university hospital of bern
helmut horten stiftung, swiss cancer research foundation
science foundation ireland through future research leaders award
Universität Bern
Publisher
Springer Science and Business Media LLC
Cited by
2 articles.
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